Multiplexed imaging of high-density libraries of RNAs with MERFISH and expansion microscopy.

ABSTRACT: As an image-based single-cell transcriptomics approach, multiplexed error-robust fluorescence in situ hybridization (MERFISH) allows hundreds to thousands of RNA species to be identified, counted and localized in individual cells while preserving the native spatial context of RNAs. In MERFISH, RNAs are identified via a combinatorial labeling approach that encodes RNA species with error-robust barcodes followed by sequential rounds of single-molecule FISH (smFISH) to read out these barcodes. The accuracy of RNA identification relies on spatially separated signals from individual RNA molecules, which limits the density of RNAs that can be measured and makes the multiplexed imaging of a large number of high-abundance RNAs challenging. Here we report an approach that combines MERFISH and expan

SUBMITTER: Wang G 

PROVIDER: S-EPMC5859009 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature