The MerR-like protein BldC binds DNA direct repeats as cooperative multimers to regulate Streptomyces development.

ABSTRACT: Streptomycetes are notable for their complex life cycle and production of most clinically important antibiotics. A key factor that controls entry into development and the onset of antibiotic production is the 68-residue protein, BldC. BldC is a putative DNA-binding protein related to MerR regulators, but lacks coiled-coil dimerization and effector-binding domains characteristic of classical MerR proteins. Hence, the molecular function of the protein has been unclear. Here we show that BldC is indeed a DNA-binding protein and controls a regulon that includes other key developmental regulators. Intriguingly, BldC DNA-binding sites vary significantly in length. Our BldC-DNA structures explain this DNA-binding capability by revealing that BldC utilizes a DNA-binding mode distinct from MerR and

SUBMITTER: Schumacher MA 

PROVIDER: S-EPMC5859096 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature