Biomimetic artificial organelles with in vitro and in vivo activity triggered by reduction in microenvironment.

ABSTRACT: Despite tremendous efforts to develop stimuli-responsive enzyme delivery systems, their efficacy has been mostly limited to in vitro applications. Here we introduce, by using an approach of combining biomolecules with artificial compartments, a biomimetic strategy to create artificial organelles (AOs) as cellular implants, with endogenous stimuli-triggered enzymatic activity. AOs are produced by inserting protein gates in the membrane of polymersomes containing horseradish peroxidase enzymes selected as a model for natures own enzymes involved in the redox homoeostasis. The inserted protein gates are engineered by attaching molecular caps to genetically modified channel porins in order to induce redox-responsive control of the molecular flow through the membrane. AOs preserve their structu

SUBMITTER: Einfalt T 

PROVIDER: S-EPMC5859287 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature