Does heterogeneity matter in the estimation of tumour budding and tumour stroma ratio in colon cancer?

ABSTRACT:

Background

Tumour budding (TB) and Tumour Stroma Ratio (TSR) may be rewarding in the treatment stratification of patients with stage II colon cancer. However, lack of standardization may exclude these parameters from being used in a clinical setting. The purpose of this methodologic study was to compare stereology with semi-quantitative estimations of TSR, to investigate the intra-tumoural heterogeneity of TB and TSR, and to assess the intra- and inter-observer agreement.

Methods

Three paraffin embedded tumour blocks, one of them representing the deepest invasive front, were selected from each of 43 patients treated for stage II colon cancer. TSR was estimated in H&E sections semi-quantitatively using conventional microscopy, and stereologically on scanned slides, using the

SUBMITTER: Eriksen AC 

PROVIDER: S-EPMC5859415 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature