Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming.

ABSTRACT:

Background

While a shift towards non-viral and animal component-free methods of generating induced pluripotent stem (iPS) cells is preferred for safer clinical applications, there is still a shortage of reliable cell sources and protocols for efficient reprogramming.

Methods

Here, we show a robust episomal and xeno-free reprogramming strategy for human iPS generation from dental pulp stem cells (DPSCs) which renders good efficiency (0.19%) over a short time frame (13-18 days).

Results

The robustness of DPSCs as starting cells for iPS induction is found due to their exceptional inherent stemness properties, developmental origin from neural crest cells, specification for tissue commitment, and differentiation capability. To investigate the epigenetic basis for the high

SUBMITTER: Thekkeparambil Chandrabose S 

PROVIDER: S-EPMC5859503 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature