Endoplasmic reticulum stress induced by an ethanol extract of Coicis semen in Chang liver cells.

ABSTRACT:

Background

It is well known that endoplasmic reticulum (ER) stress plays a huge role in development of metabolic diseases. Specially, ER stress-induced cellular dysfunction has a significant involvement in the pathogenesis of human chronic disorders. This study was designed to study to assess whether an ethanol extract of Coicis Semen (CSE) and coixol induces the ER stress in Chang liver cells.

Methods

Coicis Semen was mixed with 95% ethanol at a ratio of 1:10 (w/v) and freeze dried. Chang liver cells were seeded to 96-well plates and treated with or without CSE (100, 200, 300, 500, or 1000 μg/mL) or coixol (100, 200, 300, 500, 750, or 1000 μg/mL). cell viability was analyzed with MTT assay. Effects of CSE and coixol on expression of the genes for ER stress markers were dete

SUBMITTER: Kim HY 

PROVIDER: S-EPMC5859727 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature