Image-based correction of continuous and discontinuous non-planar axial distortion in serial section microscopy.

ABSTRACT:

Motivation

Serial section microscopy is an established method for detailed anatomy reconstruction of biological specimen. During the last decade, high resolution electron microscopy (EM) of serial sections has become the de-facto standard for reconstruction of neural connectivity at ever increasing scales (EM connectomics). In serial section microscopy, the axial dimension of the volume is sampled by physically removing thin sections from the embedded specimen and subsequently imaging either the block-face or the section series. This process has limited precision leading to inhomogeneous non-planar sampling of the axial dimension of the volume which, in turn, results in distorted image volumes. This includes that section series may be collected and imaged in unknown order.

Resul

SUBMITTER: Hanslovsky P 

PROVIDER: S-EPMC5860381 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature