T_H1 signatures are present in the lower airways of children with severe asthma, regardless of allergic status.

ABSTRACT:

Background

The pathogenesis of severe asthma in childhood remains poorly understood.

Objective

We sought to construct the immunologic landscape in the airways of children with severe asthma.

Methods

Comprehensive analysis of multiple cell types and mediators was performed by using flow cytometry and a multiplex assay with bronchoalveolar lavage (BAL) specimens (n = 68) from 52 highly characterized allergic and nonallergic children (0.5-17 years) with severe treatment-refractory asthma. Multiple relationships were tested by using linear mixed-effects modeling.

Results

Memory CCR5⁺ T_H1 cells were enriched in BAL fluid versus blood, and pathogenic respiratory viruses and bacteria were readily detected. IFN-γ⁺IL-17⁺ and I

SUBMITTER: Wisniewski JA 

PROVIDER: S-EPMC5860937 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature