Microparticles formed during storage of red blood cell units support thrombin generation.

ABSTRACT: BACKGROUND:Intact red blood cells (RBCs) appear to support thrombin generation in in vitro models of blood coagulation. During storage of RBC units, biochemical, structural, and physiological changes occur including alterations to RBC membranes and release of microparticles, which are collectively known as storage lesion. The clinical consequences of microparticle formation in RBC units are unclear. This study was performed to assess thrombin generation via the prothrombinase complex by washed RBCs and RBC-derived microparticles as a function of RBC unit age. METHODS:Well-characterized kinetic and flow cytometric assays were used to quantify and characterize microparticles isolated from leukocyte-reduced RBC units during storage for 42 days under standard blood banking conditions. RESULTS:

SUBMITTER: Bouchard BA 

PROVIDER: S-EPMC5860947 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature