Application of Large-Scale Aptamer-Based Proteomic Profiling to Planned Myocardial Infarctions.

ABSTRACT:

Background

Emerging proteomic technologies using novel affinity-based reagents allow for efficient multiplexing with high-sample throughput. To identify early biomarkers of myocardial injury, we recently applied an aptamer-based proteomic profiling platform that measures 1129 proteins to samples from patients undergoing septal alcohol ablation for hypertrophic cardiomyopathy, a human model of planned myocardial injury. Here, we examined the scalability of this approach using a markedly expanded platform to study a far broader range of human proteins in the context of myocardial injury.

Methods

We applied a highly multiplexed, expanded proteomic technique that uses single-stranded DNA aptamers to assay 4783 human proteins (4137 distinct human gene targets) to derivation and v

SUBMITTER: Jacob J 

PROVIDER: S-EPMC5860961 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature