A transgenic zebrafish model for the in vivo study of the blood and choroid plexus brain barriers using claudin 5.

ABSTRACT: The central nervous system (CNS) has specific barriers that protect the brain from potential threats and tightly regulate molecular transport. Despite the critical functions of the CNS barriers, the mechanisms underlying their development and function are not well understood, and there are very limited experimental models for their study. Claudin 5 is a tight junction protein required for blood brain barrier (BBB) and, probably, choroid plexus (CP) structure and function in vertebrates. Here, we show that the gene claudin 5a is the zebrafish orthologue with high fidelity expression, in the BBB and CP barriers, that demonstrates the conservation of the BBB and CP between humans and zebrafish. Expression of claudin 5a correlates with developmental tightening of the BBB and is restricted to a

SUBMITTER: van Leeuwen LM 

PROVIDER: S-EPMC5861362 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature