UDiTaS™, a genome editing detection method for indels and genome rearrangements.

ABSTRACT:

Background

Understanding the diversity of repair outcomes after introducing a genomic cut is essential for realizing the therapeutic potential of genomic editing technologies. Targeted PCR amplification combined with Next Generation Sequencing (NGS) or enzymatic digestion, while broadly used in the genome editing field, has critical limitations for detecting and quantifying structural variants such as large deletions (greater than approximately 100 base pairs), inversions, and translocations.

Results

To overcome these limitations, we have developed a Uni-Directional Targeted Sequencing methodology, UDiTaS, that is quantitative, removes biases associated with variable-length PCR amplification, and can measure structural changes in addition to small insertion and deletion even

SUBMITTER: Giannoukos G 

PROVIDER: S-EPMC5861650 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature