Functional Expression of the Clostridium ljungdahlii Acetyl-Coenzyme A Synthase in Clostridium acetobutylicum as Demonstrated by a Novel In Vivo CO Exchange Activity En Route to Heterologous Installation of a Functional Wood-Ljungdahl Pathway.

ABSTRACT: Engineering the Wood-Ljungdahl pathway (WLP) in the established industrial organism Clostridium acetobutylicum would allow for the conversion of carbohydrates into butanol, acetone, and other metabolites at higher yields than are currently possible, while minimizing CO2 and H2 release. To this effect, we expressed 11 Clostridium ljungdahlii core genes coding for enzymes and accessory proteins of the WLP in Clostridium acetobutylicum The engineered WLP in C. acetobutylicum showed functionality of the eastern branch of the pathway based on the formation of labeled 5,10-methylenetetrahydrofolate from 13C-labeled formate, as well as functionality of the western branch as evidenced by the formation of CO from CO2 However, the lack of labeling in acetate and butyrate pools indicated that the con

SUBMITTER: Fast AG 

PROVIDER: S-EPMC5861816 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature