Integrative single-cell omics analyses reveal epigenetic heterogeneity in mouse embryonic stem cells.

ABSTRACT: Embryonic stem cells (ESCs) consist of a population of self-renewing cells displaying extensive phenotypic and functional heterogeneity. Research towards the understanding of the epigenetic mechanisms underlying the heterogeneity among ESCs is still in its initial stage. Key issues, such as how to identify cell-subset specifically methylated loci and how to interpret the biological meanings of methylation variations remain largely unexplored. To fill in the research gap, we implemented a computational pipeline to analyze single-cell methylome and to perform an integrative analysis with single-cell transcriptome data. According to the origins of variation in DNA methylation, we determined the genomic loci associated with allelic-specific methylation or asymmetric DNA methylation, and explor

SUBMITTER: Luo Y 

PROVIDER: S-EPMC5862410 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature