Selenoprotein K deficiency inhibits melanoma by reducing calcium flux required for tumor growth and metastasis.

ABSTRACT: Interest has emerged in the therapeutic potential of inhibiting store operated calcium (Ca²⁺) entry (SOCE) for melanoma and other cancers because malignant cells exhibit a strong dependence on Ca²⁺ flux for disease progression. We investigated the effects of deleting Selenoprotein K (SELENOK) in melanoma since previous work in immune cells showed SELENOK was required for efficient Ca²⁺ flux through the endoplasmic reticulum Ca²⁺ channel protein, inositol 1,4,5-trisphosphate receptor (IP3R), which is due to the role SELENOK plays in palmitoylating and stabilizing the expression of IP3R. CRISPR/Cas9 was used to generate SELENOK-deficiency in human melanoma cells and this led to reduced Ca²⁺ flux and impaired IP3R function, which inhibite

SUBMITTER: Marciel MP 

PROVIDER: S-EPMC5862587 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature