Inter-assay precision of clonogenic assays for radiosensitivity in cancer cell line A549.

ABSTRACT: Clonogenic assays are the gold standard for determining radiosensitivity, which governs tumor response to radiation therapy. Although multiple studies of clonogenic assays on cancer cell lines have been published, the robustness of this technique has not been examined by comparative analysis of data from different studies. To address this issue, we investigated the inter-assay precision of clonogenic assays by analyzing in-house and published data on A549, a cell line frequently studied in this context. The coefficients of variation for SF2, the surviving fraction after 2 Gy irradiation, and D10, the radiation dose that reduces survival to 10%, were below 30% for both in-house data obtained from 20 independent experiments performed under consistent experimental settings (i.e., radiation ty

SUBMITTER: Nuryadi E 

PROVIDER: S-EPMC5862609 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature