Exosomal microRNA profiling to identify hypoxia-related biomarkers in prostate cancer.

ABSTRACT: Hypoxia and expression of hypoxia-related biomarkers are associated with disease progression and treatment failure in prostate cancer (PCa). We have reported that exosomes (nanovesicles of 30-150 nm in diameter) secreted by human PCa cells under hypoxia promote invasiveness and stemness in naïve PCa cells. Here, we identified the unique microRNAs (miRNAs) loaded in exosomes secreted by PCa cells under hypoxia. Using TaqMan^® array microRNA cards, we analyzed the miRNA profile in exosomes secreted by human PCa LNCaP cells under hypoxic (Exo^Hypoxic) and normoxic (Exo^Normoxic) conditions. We identified 292 miRNAs loaded in both Exo^Hypoxic and Exo^Normoxic. The top 11 miRNAs with significantly higher level in Exo^Hypoxic compared t

SUBMITTER: Panigrahi GK 

PROVIDER: S-EPMC5862624 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature