YcfD_RM is a thermophilic oxygen-dependent ribosomal protein uL16 oxygenase.

ABSTRACT: YcfD from Escherichia coli is a homologue of the human ribosomal oxygenases NO66 and MINA53, which catalyse histidyl-hydroxylation of the 60S subunit and affect cellular proliferation (Ge et al., Nat Chem Biol 12:960-962, 2012). Bioinformatic analysis identified a potential homologue of ycfD in the thermophilic bacterium Rhodothermus marinus (ycfD_RM). We describe studies on the characterization of ycfD_RM, which is a functional 2OG oxygenase catalysing (2S,3R)-hydroxylation of the ribosomal protein uL16 at R82, and which is active at significantly higher temperatures than previously reported for any other 2OG oxygenase. Recombinant ycfD_RM manifests high thermostability (T_m 84 °C) and activity at higher temperatures (T_opt 55 °C) than ycf

SUBMITTER: Sekirnik R 

PROVIDER: S-EPMC5862935 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature