Flexible pseudotyping of retrovirus using recombinase-mediated cassette exchange.

ABSTRACT:

Objective

Develop an engineered cell line containing two flexible gene expression systems enabling the continuous production of tailor-made recombinant gammaretrovirus with predictable productivities through targeted integration.

Results

Dual-FLEX cells (dFLEX) contain two independent recombinase-mediated cassette exchange (RMCE) systems which confer flexibility to the expression of different transgene and envelope combinations. The flexible envelope expression in dFLEX cells was validated by pseudotyping retrovirus particles with three different viral envelope proteins-GaLV, 4070A and VSV-G. Our results show that dFLEX cells are able to provide high titers of infectious retroviral particles with a single-copy integration of the envelope constructs after RMCE. The integrated

SUBMITTER: Soares HR 

PROVIDER: S-EPMC5862940 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature