Talaromyces marneffei simA Encodes a Fungal Cytochrome P450 Essential for Survival in Macrophages.

ABSTRACT: Fungi are adept at occupying specific environmental niches and often exploit numerous secondary metabolites generated by the cytochrome P450 (CYP) monoxygenases. This report describes the characterization of a yeast-specific CYP encoded by simA ("survival in macrophages"). Deletion of simA does not affect yeast growth at 37°C in vitro but is essential for yeast cell production during macrophage infection. The ΔsimA strain exhibits reduced conidial germination and intracellular growth of yeast in macrophages, suggesting that the enzymatic product of SimA is required for normal fungal growth in vivo. Intracellular ΔsimA yeast cells exhibit cell wall defects, and metabolomic and chemical sensitivity data suggest that SimA may promote chitin synthesis

SUBMITTER: Boyce KJ 

PROVIDER: S-EPMC5863032 | biostudies-literature | 2018 Mar-Apr

REPOSITORIES: biostudies-literature