Short Communication: Anatomic Site of Sampling and the Rectal Mucosal Microbiota in HIV Negative Men Who Have Sex with Men Engaging in Condomless Receptive Anal Intercourse.

ABSTRACT: Rectal transmission accounts for the majority of HIV transmissions among men who have sex with men (MSM). We previously demonstrated a distinct rectal mucosal immune environment in MSM engaging in condomless receptive anal intercourse (CRAI) compared with men who do not engage in anal intercourse, including enrichment of the microbiota for the Prevotellaceae family as compared with Bacteroidaceae. Here, we expand upon these previous findings to determine differences by anatomic site of collection (anal vs. rectal mucosa) and examine associations of the predominant taxa with other genera. We analyzed 16SrRNA gene sequences of the V1-V2 region generated on an Illumina MiSeq® from 35 MSM engaging in CRAI and 20 male controls. Observation by principal coordinates analysis and analysis of simil

SUBMITTER: Pescatore NA 

PROVIDER: S-EPMC5863096 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature