A robust mass spectrometry method for rapid profiling of erythrocyte ghost membrane proteomes.

ABSTRACT:

Background

Red blood cell (RBC) physiology is directly linked to many human disorders associated with low tissue oxygen levels or anemia including chronic obstructive pulmonary disease, congenital heart disease, sleep apnea and sickle cell anemia. Parasites such as Plasmodium spp. and phylum Apicomplexa directly target RBCs, and surface molecules within the RBC membrane are critical for pathogen interactions. Proteomics of RBC membrane 'ghost' fractions has therefore been of considerable interest, but protocols described to date are either suboptimal or too extensive to be applicable to a larger set of clinical cohorts.

Methods

Here, we describe an optimised erythrocyte isolation protocol from blood, tested for various storage conditions and explored using diff

SUBMITTER: Fye HKS 

PROVIDER: S-EPMC5863380 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature