Peptide/Cas9 nanostructures for ribonucleoprotein cell membrane transport and gene edition.

ABSTRACT: The discovery of RNA guided endonucleases has emerged as one of the most important tools for gene edition and biotechnology. The selectivity and simplicity of the CRISPR/Cas9 strategy allows the straightforward targeting and editing of particular loci in the cell genome without the requirement of protein engineering. However, the transfection of plasmids encoding the Cas9 and the guide RNA could lead to undesired permanent recombination and immunogenic responses. Therefore, the direct delivery of transient Cas9 ribonucleoprotein constitutes an advantageous strategy for gene edition and other potential therapeutic applications of the CRISPR/Cas9 system. The covalent fusion of Cas9 with penetrating peptides requires multiple incubation steps with the target cells to achieve efficient levels

SUBMITTER: Lostale-Seijo I 

PROVIDER: S-EPMC5863697 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature