Growth differentiation factor 15 contributes to marrow adipocyte remodeling in response to the growth of leukemic cells.

ABSTRACT:

Background

The adipocyte remodeling, including of the morphological change, might indicate special pathological function. Our previous study found that the morphological remodeling of larger marrow adipocytes into small marrow adipocytes correlates with a poor prognosis for acute myeloid leukemia (AML) patients. However, the mechanisms contributed to the marrow adipocyte remodeling are still poorly understood.

Methods

GDF15 expression was analyzed by RT-qPCR and western blotting assays in the leukemic cells. The enhancing and antibody neutralization tests in vitro were employed to evaluate the effect of GDF15 on the morphology of mature adipocytes. CCK8 test was used to detect the proliferation of leukemic cells after co-cultivation with small marrow adipocytes. Flow cytomet

SUBMITTER: Lu W 

PROVIDER: S-EPMC5863796 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature