PHD-finger domain protein 5A functions as a novel oncoprotein in lung adenocarcinoma.

ABSTRACT:

Background

PHD-finger domain protein 5A (PHF5A) is a highly conserved small transcriptional regulator also involved in pre-mRNA splicing; however, its biological functions and molecular mechanisms in non-small cell lung cancer (NSCLC) have not yet been investigated. The purpose of this study was to determine the functional relevance and therapeutic potential of PHF5A in lung adenocarcinoma (LAC).

Methods

The expression of PHF5A in LAC tissues and adjacent non-tumor (ANT) tissues was investigated using immunohistochemistry of a tissue microarray, qRT-PCR, western blot and bioinformatics. The function of PHF5A was determined using several in vitro assays and also in vivo assay by lentiviral vector-mediated PHF5A depletion in LAC cell lines.

Results

PHF5A was highly upre

SUBMITTER: Yang Y 

PROVIDER: S-EPMC5863814 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature