Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNFα production in CHO cells.

ABSTRACT: Chinese hamster ovary (CHO) cells are the most frequently used host for commercial production of therapeutic proteins. However, their low protein productivity in culture is the main hurdle to overcome. Mild hypothermia has been established as an effective strategy to enhance protein specific productivity, although the causes of such improvement still remain unclear. The self-regulation of global transcriptional regulatory factors, such as Myc and XBP1s, seems to be involved in increased the recombinant protein production at low temperature. This study evaluated the impact of low temperature in CHO cell cultures on myc and xbp1s expression and their effects on culture performance and cell metabolism. Two anti-TNFα producing CHO cell lines were selected considering two distinct phenotypes: i

SUBMITTER: Torres M 

PROVIDER: S-EPMC5864046 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature