Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors.

ABSTRACT: The ability to remotely trigger CRISPR/Cas9 activity would enable new strategies to study cellular events with greater precision and complexity. In this work, we have developed a method to photocage the activity of the guide RNA called "CRISPR-plus" (CRISPR-precise light-mediated unveiling of sgRNAs). The photoactivation capability of our CRISPR-plus method is compatible with the simultaneous targeting of multiple DNA sequences and supports numerous modifications that can enable guide RNA labeling for use in imaging and mechanistic investigations.

SUBMITTER: Jain PK 

PROVIDER: S-EPMC5864249 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature