Engineering a multicellular vascular niche to model hematopoietic cell trafficking.

ABSTRACT:

Background

The marrow microenvironment and vasculature plays a critical role in regulating hematopoietic cell recruitment, residence, and maturation. Extensive in vitro and in vivo studies have aimed to understand the marrow cell types that contribute to hematopoiesis and the stem cell environment. Nonetheless, in vitro models are limited by a lack of complex multicellular interactions, and cellular interactions are not easily manipulated in vivo. Here, we develop an engineered human vascular marrow niche to examine the three-dimensional cell interactions that direct hematopoietic cell trafficking.

Methods

Using soft lithography and injection molding techniques, fully endothelialized vascular networks were fabricated in type I collagen matrix, and co-cultured under flow with

SUBMITTER: Kotha SS 

PROVIDER: S-EPMC5865379 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature