Lipopolysaccharide suppresses IgE-mast cell-mediated reactions.

ABSTRACT:

Background

Clinical and experimental analyses have identified a central role for IgE/FcεRI/mast cells in promoting IgE-mediated anaphylaxis. Recent data from human studies suggest that bacterial infections can alter susceptibility to anaphylaxis.

Objective

We examined the effect of LPS exposure on the induction of IgE-mast cell (MC) mediated reactions in mice.

Methods

C57BL/6 WT, tlr4^-/- and IL10^-/- mice were exposed to LPS, and serum cytokines (TNF and IL-10) were measured. Mice were subsequently treated with anti-IgE, and the symptoms of passive IgE-mediated anaphylaxis, MC activation, Ca²⁺ -mobilization and the expression of FcεRI on peritoneal MCs were quantitated.

Results

We show that LPS exposure of C57BL/6 WT mice constra

SUBMITTER: Wang N 

PROVIDER: S-EPMC5865592 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature