Luminal breast cancer-specific circular RNAs uncovered by a novel tool for data analysis.

ABSTRACT: Circular RNAs are highly stable molecules present in all eukaryotes generated by distinct transcript processing. We have exploited poly(A-) RNA-Seq data generated in our lab in MCF-7 breast cancer cells to define a compilation of exonic circRNAs more comprehensive than previously existing lists. Development of a novel computational tool, named CircHunter, allowed us to more accurately characterize circRNAs and to quantitatively evaluate their expression in publicly available RNA-Seq data from breast cancer cell lines and tumor tissues. We observed and confirmed, by ChIP analysis, that exons involved in circularization events display significantly higher levels of the histone post-transcriptional modification H3K36me3 than non-circularizing exons. This result has potential impact on

SUBMITTER: Coscujuela Tarrero L 

PROVIDER: S-EPMC5865691 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature