Ribosome profiling reveals changes in translational status of soybean transcripts during immature cotyledon development.

ABSTRACT: To understand translational capacity on a genome-wide scale across three developmental stages of immature soybean seed cotyledons, ribosome profiling was performed in combination with RNA sequencing and cluster analysis. Transcripts representing 216 unique genes demonstrated a higher level of translational activity in at least one stage by exhibiting higher translational efficiencies (TEs) in which there were relatively more ribosome footprint sequence reads mapping to the transcript than were present in the control total RNA sample. The majority of these transcripts were more translationally active at the early stage of seed development and included 12 unique serine or cysteine proteases and 16 2S albumin and low molecular weight cysteine-rich proteins that may serve as substrates for tur

SUBMITTER: Shamimuzzaman M 

PROVIDER: S-EPMC5865733 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature