Artificial Metalloproteins Containing Co₄O₄ Cubane Active Sites.

ABSTRACT: Artificial metalloproteins (ArMs) containing Co₄O₄ cubane active sites were constructed via biotin-streptavidin technology. Stabilized by hydrogen bonds (H-bonds), terminal and cofacial Co^III-OH₂ moieties are observed crystallographically in a series of immobilized cubane sites. Solution electrochemistry provided correlations of oxidation potential and pH. For variants containing Ser and Phe adjacent to the metallocofactor, 1e^-/1H⁺ chemistry predominates until pH 8, above which the oxidation becomes pH-independent. Installation of Tyr proximal to the Co₄O₄ active site provided a single H-bond to one of a set of cofacial Co^III-OH₂ groups. With this variant, multi-e^-/multi

SUBMITTER: Olshansky L 

PROVIDER: S-EPMC5866047 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature