Preparation and applications of guard cell protoplasts from the leaf epidermis of Solanum lycopersicum.

ABSTRACT:

Background

Guard cell protoplasts (GCPs) isolated from various plants have proven to be especially useful for studies of signal transduction pathways and plant development. But it is not easy to isolate highly purified preparations of large numbers of GCPs from plants. In this research, our focus is on a method to isolate large numbers of guard cells from tomato leaves. The protocols described yield millions of highly purified, viable GCPs, which are also suitable for studies on guard cell physiology.

Results

We developed an efficient method for isolating GCPs from epidermal fragments of tomato leaves. The protocol requires a two-step digestion to isolate high-quality tomato GCPs. In this procedure, cellulysin (in method L) was replaced by cellulose "Onozuka" RS (in method S

SUBMITTER: Yao X 

PROVIDER: S-EPMC5866509 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature