Layered dynamic regulation for improving metabolic pathway productivity in Escherichia coli.

ABSTRACT: Microbial production of value-added chemicals from biomass is a sustainable alternative to chemical synthesis. To improve product titer, yield, and selectivity, the pathways engineered into microbes must be optimized. One strategy for optimization is dynamic pathway regulation, which modulates expression of pathway-relevant enzymes over the course of fermentation. Metabolic engineers have used dynamic regulation to redirect endogenous flux toward product formation, balance the production and consumption rates of key intermediates, and suppress production of toxic intermediates until later in the fermentation. Most cases, however, have utilized a single strategy for dynamically regulating pathway fluxes. Here we layer two orthogonal, autonomous, and tunable dynamic regulation strategies to

SUBMITTER: Doong SJ 

PROVIDER: S-EPMC5866568 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature