Single-molecule analysis of phospholipid scrambling by TMEM16F.

ABSTRACT: Transmembrane protein 16F (TMEM16F) is a Ca²⁺-dependent phospholipid scramblase that translocates phospholipids bidirectionally between the leaflets of the plasma membrane. Phospholipid scrambling of TMEM16F causes exposure of phosphatidylserine in activated platelets to induce blood clotting and in differentiated osteoblasts to promote bone mineralization. Despite the importance of TMEM16F-mediated phospholipid scrambling in various biological reactions, the fundamental features of the scrambling reaction remain elusive due to technical difficulties in the preparation of a platform for assaying scramblase activity in vitro. Here, we established a method to express and purify mouse TMEM16F as a dimeric molecule by constructing a stable cell line and developed a microarray contai

SUBMITTER: Watanabe R 

PROVIDER: S-EPMC5866571 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature