Conformational transitions of the sodium-dependent sugar transporter, vSGLT.

ABSTRACT: Sodium-dependent transporters couple the flow of Na⁺ ions down their electrochemical potential gradient to the uphill transport of various ligands. Many of these transporters share a common core structure composed of a five-helix inverted repeat and deliver their cargo utilizing an alternating-access mechanism. A detailed characterization of inward-facing conformations of the Na⁺-dependent sugar transporter from Vibrio parahaemolyticus (vSGLT) has previously been reported, but structural details on additional conformations and on how Na⁺ and ligand influence the equilibrium between other states remains unknown. Here, double electron-electron resonance spectroscopy, structural modeling, and molecular dynamics are utilized to deduce ligand-dependent eq

SUBMITTER: Paz A 

PROVIDER: S-EPMC5866573 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature