Conserved in situ arrangement of complex I and III₂ in mitochondrial respiratory chain supercomplexes of mammals, yeast, and plants.

ABSTRACT: We used electron cryo-tomography and subtomogram averaging to investigate the structure of complex I and its supramolecular assemblies in the inner mitochondrial membrane of mammals, fungi, and plants. Tomographic volumes containing complex I were averaged at ∼4 nm resolution. Principal component analysis indicated that ∼60% of complex I formed a supercomplex with dimeric complex III, while ∼40% were not associated with other respiratory chain complexes. The mutual arrangement of complex I and III₂ was essentially conserved in all supercomplexes investigated. In addition, up to two copies of monomeric complex IV were associated with the complex I₁III₂ assembly in bovine heart and the yeast Yarrowia lipolytica, but their positions varied. No complex I

SUBMITTER: Davies KM 

PROVIDER: S-EPMC5866595 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature