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Necessity of electrically conductive pili for methanogenesis with magnetite stimulation.


ABSTRACT:

Background

Magnetite-mediated direct interspecies electron transfer (DIET) between Geobacter and Methanosarcina species is increasingly being invoked to explain magnetite stimulation of methane production in anaerobic soils and sediments. Although magnetite-mediated DIET has been documented in defined co-cultures reducing fumarate or nitrate as the electron acceptor, the effects of magnetite have only been inferred in methanogenic systems.

Methods

Concentrations of methane and organic acid were analysed with a gas chromatograph and high-performance liquid chromatography, respectively. The concentration of HCl-extractable Fe(II) was determined by the ferrozine method. The association of the defined co-cultures of G. metallireducens and M. barkeri w

SUBMITTER: Wang O 

PROVIDER: S-EPMC5866715 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature