Determination of protein oligomeric structure from small-angle X-ray scattering.

ABSTRACT: Small-angle X-ray scattering (SAXS) is useful for determining the oligomeric states and quaternary structures of proteins in solution. The average molecular mass in solution can be calculated directly from a single SAXS curve collected on an arbitrary scale from a sample of unknown protein concentration without the need for beamline calibration or protein standards. The quaternary structure in solution can be deduced by comparing the experimental SAXS curve to theoretical curves calculated from proposed models of the oligomer. This approach is especially robust when the crystal structure of the target protein is known, and the candidate oligomer models are derived from the crystal lattice. When SAXS data are obtained at multiple protein concentrations, this analysis can provide insight int

SUBMITTER: Korasick DA 

PROVIDER: S-EPMC5866936 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature