Development and evaluation of high-density Axiom^® CicerSNP Array for high-resolution genetic mapping and breeding applications in chickpea.

ABSTRACT: To accelerate genomics research and molecular breeding applications in chickpea, a high-throughput SNP genotyping platform 'Axiom^® CicerSNP Array' has been designed, developed and validated. Screening of whole-genome resequencing data from 429 chickpea lines identified 4.9 million SNPs, from which a subset of 70 463 high-quality nonredundant SNPs was selected using different stringent filter criteria. This was further narrowed down to 61 174 SNPs based on p-convert score ≥0.3, of which 50 590 SNPs could be tiled on array. Among these tiled SNPs, a total of 11 245 SNPs (22.23%) were from the coding regions of 3673 different genes. The developed Axiom^® CicerSNP Array was used for genotyping two recombinant inbred line populations, namely ICCRIL03 (ICC 4958 × ICC 1882) a

SUBMITTER: Roorkiwal M 

PROVIDER: S-EPMC5866945 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature