IL-21 promotes myocardial ischaemia/reperfusion injury through the modulation of neutrophil infiltration.

ABSTRACT:

Background and purpose

The immune system plays an important role in driving the acute inflammatory response following myocardial ischaemia/reperfusion injury (MIRI). IL-21 is a pleiotropic cytokine with multiple immunomodulatory effects, but its role in MIRI is not known.

Experimental approach

Myocardial injury, neutrophil infiltration and the expression of neutrophil chemokines KC (CXCL1) and MIP-2 (CXCL2) were studied in a mouse model of MIRI. Effects of IL-21 on the expression of KC and MIP-2 in neonatal mouse cardiomyocytes (CMs) and cardiac fibroblasts (CFs) were determined by real-time PCR and ELISA. The signalling mechanisms underlying these effects were explored by western blot analysis.

Key results

IL-21 was elevated within the acute phase of murine MIRI. Neu

SUBMITTER: Wang K 

PROVIDER: S-EPMC5866974 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature