Rapid evolution of a bacterial iron acquisition system.

ABSTRACT: Under iron limitation, bacteria scavenge ferric (Fe³⁺ ) iron bound to siderophores or other chelates from the environment to fulfill their nutritional requirement. In gram-negative bacteria, the siderophore uptake system prototype consists of an outer membrane transporter, a periplasmic binding protein and a cytoplasmic membrane transporter, each specific for a single ferric siderophore or siderophore family. Here, we show that spontaneous single gain-of-function missense mutations in outer membrane transporter genes of Bradyrhizobium japonicum were sufficient to confer on cells the ability to use synthetic or natural iron siderophores, suggesting that selectivity is limited primarily to the outer membrane and can be readily modified. Moreover, growth on natural or synthetic che

SUBMITTER: Chatterjee A 

PROVIDER: S-EPMC5867251 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature