Detailed Characterization of T Cell Receptor Repertoires in Multiple Sclerosis Brain Lesions.

ABSTRACT: The antigen-specific activation of pathogenic T cells is considered essential in the initiation and maintenance of multiple sclerosis (MS). The site of activation, the differential involvement of CD4+, and CD8+ T cells, their functional phenotype, and specificity, are important aspects to understand MS pathogenesis. The analysis of clonal expansions of brain-infiltrating T cells may reveal local antigen-driven activation or specific brain homing and allow the identification of putatively pathogenic T cells. We used high-throughput T cell receptor β-chain variable gene (TRBV) sequencing (-seq) of genomic (g)DNA, which reflects the quantity and diversity of the TRBV repertoire, to characterize three white matter demyelinating lesions with different location and inflammatory activity, and pai

SUBMITTER: Planas R 

PROVIDER: S-EPMC5867461 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature