Purification and Characterization of a Novel Alginate Lyase from the Marine Bacterium Bacillus sp. Alg07.

ABSTRACT: Alginate oligosaccharides with different bioactivities can be prepared through the specific degradation of alginate by alginate lyases. Therefore, alginate lyases that can be used to degrade alginate under mild conditions have recently attracted public attention. Although various types of alginate lyases have been discovered and characterized, few can be used in industrial production. In this study, AlgA, a novel alginate lyase with high specific activity, was purified from the marine bacterium Bacillus sp. Alg07. AlgA had a molecular weight of approximately 60 kDa, an optimal temperature of 40 °C, and an optimal pH of 7.5. The activity of AlgA was dependent on sodium chloride and could be considerably enhanced by Mg²⁺ or Ca²⁺. Under optimal conditions, the act

SUBMITTER: Chen P 

PROVIDER: S-EPMC5867630 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature