Exploring native genetic elements as plug-in tools for synthetic biology in the cyanobacterium Synechocystis sp. PCC 6803.

ABSTRACT:

Background

The unicellular cyanobacterium Synechocystis sp. PCC 6803 has been widely used as a photoautotrophic host for synthetic biology studies. However, as a green chassis to capture CO₂ for biotechnological applications, the genetic toolbox for Synechocystis 6803 is still a limited factor.

Results

We systematically characterized endogenous genetic elements of Synechocystis 6803, including promoters, ribosome binding sites, transcription terminators, and plasmids. Expression from twelve native promoters was compared by measuring fluorescence from the reporter protein EYFP in an identical setup, exhibiting an 8000-fold range of promoter activities. Moreover, we measured the strength of twenty native ribosome binding sites and eight native terminators, indicatin

SUBMITTER: Liu D 

PROVIDER: S-EPMC5868059 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature