Assessment of viral methylation levels for high risk HPV types by newly designed consensus primers PCR and pyrosequencing.

ABSTRACT:

Background

Measuring viral DNA methylation in human papillomavirus (HPV) infected women showed promise for accurate detection of high-grade cervical lesions and cancer. Methylation status has been widely investigated for HPV16, sporadically for other HPV types.

Methods

Objective of this methodological study was to set up molecular methods to test the methylation levels in the twelve oncogenic HPV types by pyrosequencing, minimizing the number of HPV type-specific PCR protocols. Target CpGs were selected on the HPV L1 (two regions, L1 I and L1 II) and L2 genes. Study samples included DNA stored at Turin, Italy, purified by cervical cells collected in Standard Transport Medium or PreservCyt from women who participated in two studies (N = 126 and 140) nested within the regional

SUBMITTER: Gillio-Tos A 

PROVIDER: S-EPMC5868804 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature