A Cell Culture Approach to Optimized Human Corneal Endothelial Cell Function.

ABSTRACT:

Purpose

Cell-based therapies to replace corneal endothelium depend on culture methods to optimize human corneal endothelial cell (HCEC) function and minimize endothelial-mesenchymal transition (EnMT). Here we explore contribution of low-mitogenic media on stabilization of phenotypes in vitro that mimic those of HCECs in vivo.

Methods

HCECs were isolated from cadaveric donor corneas and expanded in vitro, comparing continuous presence of exogenous growth factors ("proliferative media") to media without those factors ("stabilizing media"). Identity based on canonical morphology and expression of surface marker CD56, and function based on formation of tight junction barriers measured by trans-endothelial electrical resistance assays (TEER) were assessed.

Results

Primary

SUBMITTER: Bartakova A 

PROVIDER: S-EPMC5869002 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature