ES cell-derived presomitic mesoderm-like tissues for analysis of synchronized oscillations in the segmentation clock.

ABSTRACT: Somites are periodically formed by segmentation of the anterior parts of the presomitic mesoderm (PSM). In the mouse embryo, this periodicity is controlled by the segmentation clock gene Hes7, which exhibits wave-like oscillatory expression in the PSM. Despite intensive studies, the exact mechanism of such synchronous oscillatory dynamics of Hes7 expression still remains to be analyzed. Detailed analysis of the segmentation clock has been hampered because it requires the use of live embryos, and establishment of an in vitro culture system would facilitate such analyses. Here, we established a simple and efficient method to generate mouse ES cell-derived PSM-like tissues, in which Hes7 expression oscillates like traveling waves. In these tissues, Hes7 osci

SUBMITTER: Matsumiya M 

PROVIDER: S-EPMC5869006 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature