Rapid and simple purification of elastin-like polypeptides directly from whole cells and cell lysates by organic solvent extraction.

ABSTRACT: Elastin-like polypeptides (ELP) are a well-known class of proteins that are being increasingly utilized in a variety of biomedical applications, due to their beneficial physicochemical properties. A unifying feature of ELP is their demonstration of a sequence tunable inverse transition temperature (Tt) that enables purification using a simple, straightforward process called inverse transition cycling (ITC). Despite the utility of ITC, the process is inherently limited to ELP with an experimentally accessible Tt. Since the underlying basis for the ELP Tt is related to its high overall hydrophobicity, we anticipated that ELP would be excellent candidates for purification by organic extraction. We report the first method for rapidly purifying ELP directly from whole E. coli cells or clarified

SUBMITTER: VerHeul R 

PROVIDER: S-EPMC5869169 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature