Analysis of the Aedes albopictus C6/36 genome provides insight into cell line utility for viral propagation.

ABSTRACT:

Background

The 50-year-old Aedes albopictus C6/36 cell line is a resource for the detection, amplification, and analysis of mosquito-borne viruses including Zika, dengue, and chikungunya. The cell line is derived from an unknown number of larvae from an unspecified strain of Aedes albopictus mosquitoes. Toward improved utility of the cell line for research in virus transmission, we present an annotated assembly of the C6/36 genome.

Results

The C6/36 genome assembly has the largest contig N50 (3.3 Mbp) of any mosquito assembly, presents the sequences of both haplotypes for most of the diploid genome, reveals independent null mutations in both alleles of the Dicer locus, and indicates a male-specific genome. Gene annotation was computed with publicly available mosquito transcr

SUBMITTER: Miller JR 

PROVIDER: S-EPMC5869287 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature